We are also making changes to equipment in all of our location. Beginning in mid June, the UFCR will be occupying 1200 sq. This same process may be performed on a cell sorting cytometer which additionally allows for the capture of subsets of the cells or particles to be re-used by the customer in a subsequent procedure.Įxciting changes are happening for UFCR with the opening of the Cancer-Cardio Research Building (CCRB), funding and key internal agreements. The result is an electronic file containing the measured information, which may be represented in a graphical format using specific software to interpret the file. Information about the size and granularity of a cell may be recorded, as well. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is output to a computer workstation. The fluorescent probes are excited by the laser and then emit light energy. The cells then pass by a series of lasers, each of which emit a specific wavelength of light. Cells or particles tagged with fluorescent molecules enter the cytometer via a fluid stream. Please let the UFCR staff know if you have any questions.įlow cytometry uses fluorescent probes to identify and characterize cells or particles. We will let everyone know when we can revert back to our normal procedures. We aim to keep everyone healthy, so we can stay productive. Wash your hands with soap and water, cover your mouth when coughing or sneezing, and do not come to school or work when you do not feel well.īy following these guidelines we hope to minimize the chances of transmission of coronavirus or any other seasonal illness between our users and staff. We encourage you to continue to follow the Minnesota Department of Health (MDH) guidance by taking the most basic measures to protect yourself-and all of us. Reminder: No food or drinks in cytometer rooms.Wipe instruments and work surfaces with sanitizing wipes or 70-80% ethanol before and after use.Wear gloves that have NOT been worn for sample or slide preparation when handling the instrument.Please send Sunrise plan AND letter of approval to Wash hands with soap and water or hand sanitizer before and after (washing stations available in each facility). Request FlowJo Site License Access Hardware Address of the computer having FlowJo installed on it (how to find HWA) The name of the PI paying for the FlowJo. Approval documentation for your lab MUST be on file with the UFCR for requests to be honored. Please use this link to request a reservation on our cell analyzer instrumentation.Many issues can and should be solved electronically to avoid unnecessary exposure to infectious agents. Contact us by email or telephone to set up an appointment. Do not show up at our offices unannounced.If you have a fever and/or are coughing/sneezing, please stay at home. We will gladly cancel any reservation at no charge due to illness.At the University Flow Cytometry Resource, dozens of people each week touch common work surfaces repeatedly (knobs, buttons, mouse, keyboard, etc.), so we have established a protocol with specific steps to minimize chances of viral transmission through this route: One established route of transmission for this virus is via contaminated surfaces. If you would like more information about the site license, please contact Michele Black at 685-3014.The coronavirus has reached MN. If your lab has a shared computer that you would like to license inorder for multiply users to access, please email and provide the following infromation: You will then get an email from the email from "FlowJo Notifications" (check your spam folder) when you accept the invitation you will be asked to provide the following information. If you would like to get a license for FlowJo you should first create a FlowJo Portal account Once you have created an account, you can email request an invitation to the site license. Researcher that would like to have their own copy of FlowJo on their personal or work computer can request a license that they will be able to self-manage up to 4 different devices that can analyze data with FlowJo (one at a time) The software is compatible with both MAC and PC's.Īnyone can create a FlowJo Portal account for free to access licensed copies of FlowJo on shared computers. An individual copy of FlowJo is normally $2695.00 with the academic discount the site license brings that cost down to $285/year for each license that can be used on up to 4 different computers (one at a time) or licensed on a shared computer that multiple users can login with their own Portal account to access FlowJo. FlowJo has launched their new username and password based licensing system called FlowJo Portal the University of Washington has access to site licensing through the Cell Analysis Facility.
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